ABSTRACT
Objetivo: O objetivo deste estudo foi avaliar as condições de saúde bucal através de parâmetros salivares e microbiológicos associados á cárie dental em pacientes com PC submetidas ou não à gastrostomia na cidade de Uberaba. Métodos: Trata-se de um estudo observacional transversal, realizado com uma amostra selecionada por conveniência com 48 pacientes com idades entre 2 anos e 16 anos, pertencentes aos Ambulatórios de Cirurgia Pediátrica e da Neurologia do Hospital de Clínicas da UFTM e do Hospital da Criança da cidade de Uberaba, divididos em 3 grupos sendo, 16 pacientes sem deficiência (Grupo SD), 15 pacientes com Paralisia Cerebral (Grupo PC) e 17 pacientes com PC submetidas à gastrostomia e fundoplicatura de Nissen (Grupo PCG). Avaliou-se o índice de cárie (IC), o índice de placa (IP), contagem salivar do número de colônias de Streptococcus mutans (CB) e a capacidade de tamponamento salivar (CTS). Os dados obtidos de IP, CB e CTS foram submetidos à análise estatística One Way Anova (p<0,05), e os dados de IP ao Teste de Tukey (p<0,05). A avaliação do IC se deu através de análise estatística descritiva da porcentagem simples das faces cariadas em relação ao número total de faces. Resultados: Observou-se diferença estatística apenas entre os grupos para o IP, sendo que o grupo PCG apresentou os maiores valores de índices de placa quando comparados aos demais. Conclusão: Concluiu-se que a PC, associada ou não à gastrostomia, não é facilitador ou atenuante da doença cárie, nem atua como fator determinante da saúde bucal.(AU)
Aim: This study sought to evaluate oral health conditions through oral clinical examinations and salivary and microbiological analyses associated with dental caries in patients with Cerebral Palsy (CP) submitted or not to gastrostomy in the city of Uberaba, MG, Brazil. Methods: This is an observational cross sectional study, conducted in a conventional sample of 48 patients, 2 to 16 years of age, at the UFTM Clinical Hospital and the Children's Hospital of Uberaba, divided into 3 groups, as follows: 16 patients without disabilities (Group WD), 15 patients with CP (Group CP), and 17 patients with CP submitted to gastrostomy and Nissen fundoplication (Group WCP). This study evaluated the caries index (CI), plaque index (PI), salivary counts of the Streptococcus mutans bacterium (CB), and salivary buffering capacity (SBC). Data from PI, CB, and SBC were statistically analyzed by the One-Way ANOVA analysis (p<0.05), and PI data were submitted to the Tukey test (p<0.05). Results: A statistically significant difference was only observed among the groups as regards the PI analysis, given that the WCP group presented the highest values. Conclusion: It was concluded that the CP, with or without gastrostomy is not a facilitator or attenuating circumstance of caries, nor is it a determining factor of good or poor oral health.(AU)
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Cerebral Palsy , Diagnosis, Oral , Oral Health , Cross-Sectional Studies , Fundoplication , Gastrostomy , Observational StudyABSTRACT
OBJETIVO: Neste trabalho, analisa-se o efeito de dieta hiperlipídico-proteica com baixo teor de carboidrato sobre o peso corporal, peso de órgãos, consumo de ração, parâmetros bioquímicos e alterações histopatológicas no fígado de ratos. MÉTODOS: Foram utilizados 24 animais - 12 no grupo-controle e 12 no grupo-experimental - com peso médio de 160 gramas no início do experimento. Semanalmente, foram verificados o peso corporal e o consumo de ração, e ao final de oito semanas foram feitas as dosagens bioquímicas sanguíneas, pesagem de órgãos e análise histopatológica dos fígados. RESULTADOS: Os animais do grupo-experimental tiveram maior ganho de peso corporal e acumularam mais tecido adiposo que os animais do grupo-controle. Fígado, rins e baço não sofreram alterações quanto ao peso. Os animais que receberam dieta hiperlipídico-proteica tiveram um aumento na ingestão energética acumulada nas oito semanas do estudo. O grupo-experimental desenvolveu hiperglicemia e hipertrigliceridemia, aumento da fração lipoproteína de alta densidade do colesterol e da creatinina sérica quando comparado ao grupo-controle. Foi detectada esteatose hepática no grupo-experimental. CONCLUSÃO: Os resultados demonstraram que dietas pobres em carboidratos e ricas em gordura e proteínas podem acarretar alterações metabólicas prejudiciais ao organismo.
OBJECTIVE: This study analyzed the effect of a high fat, high protein and low carbohydrate diet on the body weight, organ weight, food intake and biochemical parameters of rats and the histopathological changes in their livers. METHODS: A total of 24 animals were used, 12 in the control group and 12 in the experimental group, with a mean weight of 160 grams at baseline. Body weight and food intake were collected weekly. At the end of 8 weeks, the animals were killed for the biochemical tests and weighing of organs and the livers were submitted to histopathological analysis. RESULTS: The animals in the experimental group gained more weight and accumulated more body fat than the animals in the control group. The weight of the liver, kidneys and spleen did not change. The animals fed the low carbohydrate diet consumed more calories during the 8 weeks of the study period. They also developed hyperglycemia and hypertriglyceridemia and presented high high-density lipoprotein cholesterol and serum creatinine. The experimental group also presented hepatic steatosis. CONCLUSION: The results show that low carbohydrate diets that are rich in fats and proteins may result in harmful metabolic changes in rats.
Subject(s)
Animals , Rats , Dietary Carbohydrates/analysis , Diet/adverse effects , Diet/statistics & numerical data , Dietary Fats/analysis , Dietary Proteins/analysis , Rats, Wistar/metabolismABSTRACT
Aim: The aim of this study was to investigate the metabolic activity of Streptococcus mutans biofilms after treatment with mouthwashes with different compositions. Methods: S. mutans biofilms were growth on polystyrene plates during 18 h, washed with sterile saline and treated with the following mouthwashes during 1 min: Listerine®, Oral B®, Parodontax® and Periogard® with and without alcohol. After the treatment, the biofilms were incubated with complete medium containing sucrose during 60, 120 or 180 min, and then samples were collected for pH measurements. In addition, biofilms were grown in microscope coverslips treated as described above, followed by staining with Propidium Iodide and Fluoresceine for visualization with a confocal laser scanning microscopy. Results: For all mouthwashes evaluated, treatment was deleterious to cell metabolism, since little or no acidification was observed at least 60 min after treatment. Mouthwashes containing 0.2% chlorhexidine (Parodontax®) or essential oils (Listerine®) induced a significant reduction in the metabolic activity of biofilms during the tested time points (120 and 180 min after treatment), being thus more effective than the mouthwashes containing 0.12% chlorhexidine (Periogard®) or cetylpyridinium plus fluoride (Oral B®). The confocal analysis overall confirmed the results observed in the analysis of metabolic activity. Conclusions: The treatment of biofilms with mouthwashes containing 0.2% chlorhexidine or essential oils induced significant reduction in S. mutans metabolism.
Subject(s)
Biofilms , Chlorhexidine , Mouthwashes , Streptococcus mutansABSTRACT
O objetivo deste estudo foi analisar a presença de microorganismos em 50 escovas dentais em uso superior a 30 dias, imersas em solução diluída de água sanitária em tempos predeterminados, a fim de detectar qual o tempo de imersão seria eficaz na eliminação dos microorganismos presentes nas escovas dentais. Os resultados demonstraram que a imersão da região das cerdas das escovas dentais na solução diluída de água sanitária por um tempo mínimo de 8 horas é um método válido na descontaminação das cerdas impregnadas por inúmeros microorganismos, podendo ser considerado como um método sanitizante de uso doméstico
Subject(s)
Decontamination/methods , Sodium Hypochlorite , Toothbrushing , Colony Count, Microbial , Stomatitis/complications , Streptococcus mutansABSTRACT
In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pistarvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is the genetic response to ambient pH sensing in N. crassa.